THE WOKK OF FRUITS 317 How does this reach' them? A squash or a pumpkin is a favorable object for study : it may take up as much as a pound and a half of water in twenty-four hours. Cut a young growing- squash from the vine and place the cut end in eosin solution, and allow it to stand for several days. Trace the eosin up through the bundles to the seeds. Do you find a bundle reach- ing to each seed ? Does the bundle grow larger as the seed develops? Does the fruit lose water by transpi- ration? Test this matter in the fashion already described for leaves. Do you find any stomata in the epidermis of the fruit (examine microscopically and also test in the air-pump). Grapes are of especial interest in this connection: examine also apples, pears, etc. Admirable means of protection against transpiration are found in the thick, woody walls of nuts, drupes (Peach, Eucalyptus, etc.). Does the fruit need a supply of air? Repeat the experiment shown in Fig. 31, using immature fruits instead of seeds. Try the effect of smearing over young fruits completely with vaseline: does it retard their development? Do they contain much air in their tissues ? Place some of the tissue (preferably a piece of watermelon flesh) under water in the air- pump, and exhaust. How does the air travel through the stalk, etc,, to the fruit? Investigate by the methods previously described.